Immunogold Signal Amplification: Application of the CARD Approach to Electron Microscopy
نویسندگان
چکیده
منابع مشابه
Immunogold signal amplification: Application of the CARD approach to electron microscopy.
Catalyzed reporter deposition (CARD) is a technique that allows amplification of routine immunolabeling in light microscopy. This procedure takes advantage of the horseradish peroxidase (HRP) from an HRP-avidin complex to catalyze the accumulation of reporter-conjugated tyramine (a phenolic compound) onto a surface displaying biotinylated antigen-antibody complexes. The large amount of labeled ...
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15 صفحه اولImmunogold Labeling of Cultured Cells and Virus Particles for Electron Microscopy and Cryo-Electron Microscopy Applications
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We present methods for embedding brain tissue in Lowicryl K4M embedding medium and localizing antigens using postembedding immunogold techniques. After perfusion fixation with 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M cacodylate buffer, blocks of rat brain were placed in 2% aqueous uranyl acetate for 1 hour, dehydrated in 50%, 70%, and 95% ethanol, infiltrated with Lowicryl/ethanol m...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1999
ISSN: 0022-1554,1551-5044
DOI: 10.1177/002215549904700401